TPD platform

Protein degraders can induce the degradation of disease-causing proteins via the ubiquitin-proteasome or lysosomal pathways. Various classes of molecules and methods have emerged for degrader discovery, including PROTACs (proteolysis-targeting chimeras), molecular glues, and selective receptor degraders.

Biortus has developed multiple biochemical, biophysical, and cell-based assays, as well as structural determination techniques, to support protein degrader development and validation. We offer a mature pipeline for degrader development, spanning from gene analysis to assay and structural evaluation.

• Binary binding assay:

  SPR

  TR-FRET

  ELISA

  FP

  DSF

• Ternary binding assays:

  AlphaLisa/AlphaScreen

  SPR

  TR-FRET

• Target ubiquitination assays:

  WB

  TR-FRET

  AlphaLisa/AlphaScreen

  NanoBRET

• Cellular assays:

  HCS, WB for target degradation

  qPCR for mRNA level detection

  NanoBRET & NanoLuc for PPI and ubiquitination

  CETSA (split NanoLuc)