Protein

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Plasmid construction

Plasmid construction (sub-cloning) employs seamless cloning, a commonly used technique in genetic engineering. It is mainly used to insert the target gene into the vector to construct a recombinant DNA plasmid. Sub-cloning is the process of transferring a DNA fragment from one vector to another. We can achieve high-throughput for the cloning of different gene fragments into the same vector.

Service Content

The target fragment is amplified from the template according to the customer's requirements, ligated into the target vector, and verified by sequencing.

Service Description

Customer-provided information:

Template information: For the customer's original sequence, please provide the sequencing color map. If the sequence cannot be provided, sequencing verification and sequencing primer costs will be added.

Target vector information: For plasmids constructed by the customer themselves, sequencing results must be provided.

Target DNA fragment sequence.

Deliverables：

• plasmid DNA (1 μg) will be provided upon shipment.

• Corresponding sequencing results.

• Alignment files.

Prices

Vectors for Cloning

  • Mammalian

    pcDNA3.1

    pcDNA3.4

    pTT5

    pCMV5

    pEG BacMam

  • Baculovirus/Insect

    pFastBac1

    pFastBacHT-A

    pFastBacHT-B

    pFastBacHT-C

    pFastBac-Dual

  • Bacterial

    pET-11a

    pET-15b

    pET-21a

    pET-22b

    pET-24a

    pET-26b

    pET-28a

    pET-32a

    pGEX-4T-1

    pGEX-6P-1

    pMAL-c5x

    pETDuet-1

    pCDFDuet-1

Notes: For research use only.

Cloning Tags

  • N-tag

    His-TF-TEV-POI

    His-MBP-TEV-POI

    His-GST-TEV-POI

    His-TrxA-TEV-POI

    His-Gbeta-TEV-POI

    His-GFP-TEV-POI

  • C-tag

    POI-TEV-His

    POI-TEV-GFP-His

    POI-TEV-Strep II

  • Cleavage Site

    TEV

    3C

    Thrombin

    SUMO

Signal Peptide for Cloning

signal peptide

  • CD33
  • HMM
  • Mouse Ig Kappa
  • Albumin(HSA)
  • GP67
  • GP64
  • Melittin
  • HA
  • pelB