Service Process
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Evaluation & Design
Guided Design from the Beginning
Published Literature & Structures
Structure Prediction Tools
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Expression
Construct & Condition Screening
High-throughput parallel screening
Shakers, Fermenters, WAVE
E.coli, Insect Cell, Mammalian, Silkworm, etc.
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Purification
An Extension of Your Lab
Membrane, Soluble & Refolded Proteins
Standard & Specialty Columns
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Quality Control
Soluble, Consistent, Functional
Standard QC: SDS-PAGE, LC-MS, aSEC
Custom QC: nanoDSF, SPR, Negative Stain, etc.
Multiple expression systems available
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Mammalian
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General Applications
Comprehensive PTM;
Membrane proteins;
Antibodies
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Approximate Expression Time
1 week for small scale expression test;
3-5 days for expression
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Capacity
0.2 L-9.6 L/batch
200 L/week (max)
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Strains available
ExpiCHO-S, Expi293,
Expi293 GnTi-
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Insect cell
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General Applications
Simple PTMs
Scalable
Membrane proteins
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Approximate Expression Time
2 weeks for virus generation;
1 week for virus titer determination and small scale expression test;
2-3 days for expression
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Capacity
0.8 L-9.6 L/batch
400 L/week (max)
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Strains available
Sf9, Sf21, Hi-5
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E.Coli
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General Applications
Scalable
Fastest turnaround
Cost effective
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Approximate Expression Time
1 week for small scale expression test and expression optimization;
O/N expression
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Capacity
5-10 L/batch
1000 L/week(max)
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Strains available
BL21(DE3), T7 Express,
Rosetta, Artic, etc.
More than 20 choices
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Yeast
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General Applications
Low cost,
eukaryotic PTMs
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Approximate Expression Time
4-6 days
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Capacity
-
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Strains available
Pichia pastoris SMD116
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Cell free
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General Applications
High throughput;
Unnatural amino
acids(UAAs)incorporation
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Approximate Expression Time
~4 hours
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Capacity
-
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Strains available
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Silk worm
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General Applications
In vivo bioreactor
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Approximate Expression Time
2 weeks for virus generation;
~5 days after virus injection
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Capacity
~200 worms/batch
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Strains available
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Synthetic nanobody library
• Saccharomyces cerevisiae
• Library Size:5.6×10
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Screening strategies
• MACS+FACS
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Expression and purification of nanobody
• E.coli or mammalian cell
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SPR confirmation
• Kd in range of~nM
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Antigen
~1 mg (50 kD)
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Screening cycle
• 2.5-3 months
