Protein

— High Quality Drug Target Proteins

Service Process

4~8 weeks
  • Evaluation & Design

    Guided Design from the Beginning

    Published Literature & Structures
    Structure Prediction Tools
  • Expression

    Construct & Condition Screening

    High-throughput parallel screening
    Shakers, Fermenters, WAVE
    E.coli, Insect Cell, Mammalian, Silkworm, etc.
  • Purification

    An Extension of Your Lab

    Membrane, Soluble & Refolded Proteins
    Standard & Specialty Columns
  • Quality Control

    Soluble, Consistent, Functional

    Standard QC: SDS-PAGE, LC-MS, aSEC
    Custom QC: nanoDSF, SPR, Negative Stain, etc.

Multiple expression systems available

  • Mammalian

    • General Applications

      Comprehensive PTM;

      Membrane proteins;

      Antibodies

    • Approximate Expression Time

      1 week for small scale expression test;

      3-5 days for expression

    • Capacity

      0.2 L-9.6 L/batch

      200 L/week (max)

    • Strains available

      ExpiCHO-S, Expi293,

      Expi293 GnTi-

  • Insect cell

    • General Applications

      Simple PTMs

      Scalable

      Membrane proteins

    • Approximate Expression Time

      2 weeks for virus generation;

      1 week for virus titer determination and small scale expression test;

      2-3 days for expression

    • Capacity

      0.8 L-9.6 L/batch

      400 L/week (max)

    • Strains available

      Sf9, Sf21, Hi-5

  • E.Coli

    • General Applications

      Scalable

      Fastest turnaround

      Cost effective

    • Approximate Expression Time

      1 week for small scale expression test and expression optimization;

      O/N expression

    • Capacity

      5-10 L/batch

      1000 L/week(max)

    • Strains available

      BL21(DE3), T7 Express,

      Rosetta, Artic, etc.

      More than 20 choices

  • Yeast

    • General Applications

      Low cost,

      eukaryotic PTMs

    • Approximate Expression Time

      4-6 days

    • Capacity

      -

    • Strains available

      Pichia pastoris SMD116

  • Cell free

    • General Applications

      High throughput;

      Unnatural amino

      acids(UAAs)incorporation

    • Approximate Expression Time

      ~4 hours

    • Capacity

      -

    • Strains available

      -

  • Silk worm

    • General Applications

      In vivo bioreactor

    • Approximate Expression Time

      2 weeks for virus generation;

      ~5 days after virus injection

    • Capacity

      ~200 worms/batch

    • Strains available

      -

  • Synthetic nanobody library

    • Saccharomyces cerevisiae
    • Library Size:5.6×10
  • Screening strategies

    • MACS+FACS
  • Expression and purification of nanobody

    • E.coli or mammalian cell
  • SPR confirmation

    • Kd in range of~nM
  • Antigen

    ~1 mg (50 kD)
  • Screening cycle

    • 2.5-3 months

Our facilities & instruments available

• >50 AKTAs

• >250 employees in protein expression and purification department

• Dedicated membrane protein purification team

• Over 300 batches of purified protein per week

• Endotoxin removal available

• Standard columns and specialty columns available

• Nickel (NTA, Excel, etc.), GST, MBP, Flag, Protein A/G, IEX, HIC, SEC, and more.