Protein

— High Quality Drug Target Proteins

Service Process

4~8 weeks
  • Evaluation & Design

    Guided Design from the Beginning

    Published Literature & Structures
    Structure Prediction Tools
  • Expression

    Construct & Condition Screening

    High-throughput parallel screening
    Shakers, Fermenters, WAVE
    E.coli, Insect Cell, Mammalian, Silkworm, etc.
  • Purification

    An Extension of Your Lab

    Membrane, Soluble & Refolded Proteins
    Standard & Specialty Columns
  • Quality Control

    Soluble, Consistent, Functional

    Standard QC: SDS-PAGE, LC-MS, aSEC
    Custom QC: nanoDSF, SPR, Negative Stain, etc.

Multiple expression systems available

  • Mammalian

    • General Applications

      Comprehensive PTM;

      Membrane proteins;

      Antibodies

    • Approximate Expression Time

      1 week for small scale expression test;

      3-5 days for expression

    • Capacity

      0.2 L-9.6 L/batch

      200 L/week (max)

    • Strains available

      ExpiCHO-S, Expi293,

      Expi293 GnTi-

  • Insect cell

    • General Applications

      Simple PTMs

      Scalable

      Membrane proteins

    • Approximate Expression Time

      2 weeks for virus generation;

      1 week for virus titer determination and small scale expression test;

      2-3 days for expression

    • Capacity

      0.8 L-9.6 L/batch

      400 L/week (max)

    • Strains available

      Sf9, Sf21, Hi-5

  • E.Coli

    • General Applications

      Scalable

      Fastest turnaround

      Cost effective

    • Approximate Expression Time

      1 week for small scale expression test and expression optimization;

      O/N expression

    • Capacity

      5-10 L/batch

      1000 L/week(max)

    • Strains available

      BL21(DE3), T7 Express,

      Rosetta, Artic, etc.

      More than 20 choices

  • Yeast

    • General Applications

      Low cost,

      eukaryotic PTMs

    • Approximate Expression Time

      4-6 days

    • Capacity

      -

    • Strains available

      Pichia pastoris SMD116

  • Cell free

    • General Applications

      High throughput;

      Unnatural amino

      acids(UAAs)incorporation

    • Approximate Expression Time

      ~4 hours

    • Capacity

      -

    • Strains available

      -

  • Silk worm

    • General Applications

      In vivo bioreactor

    • Approximate Expression Time

      2 weeks for virus generation;

      ~5 days after virus injection

    • Capacity

      ~200 worms/batch

    • Strains available

      -

  • Synthetic nanobody library

    • Saccharomyces cerevisiae
    • Library Size:5.6×10
  • Screening strategies

    • MACS+FACS
  • Expression and purification of nanobody

    E.coli or mammalian cell
  • SPR confirmation

    • Kd in range of~nM
  • Antigen

    ~1 mg (50 kD)
  • Screening cycle

    • 2.5-3 months

Our facilities & instruments available

>50 AKTAs

>250 employees in protein expression and purification department

Dedicated membrane protein purification team

Over 300 batches of purified protein per week

Endotoxin removal available

Standard columns and specialty columns available

Nickel (NTA, Excel, etc.), GST, MBP, Flag, Protein A/G, IEX, HIC, SEC, and more.