Endoglycosidase
Glycosylation is a research hotspot in the fields of protein science and cell biology, and glycoproteins are a core research focus. The glycosylation sites on glycoproteins are often randomly distributed on the protein structure, resulting in a large degree of heterogeneity. Therefore, studying the structure of glycoproteins through endo-glycosidases has become an important method. Currently, the most widely used method for glycoprotein structural analysis is chemical sequencing.
Enzyme-based Sequencing
Chemical deglycosylation
Chemical sequencing is a method of analyzing the structure of glycoproteins by chemical reactions under specific conditions, including methods such as Smith degradation, high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance.