Universal Nuclease

In protein purification processes, nucleic acids are a common impurity component. In particular, some proteins have electrostatic binding properties, making nucleic acid removal more challenging. Currently, the main sources of nucleic acid impurities in protein samples are the nucleic acids remaining in the sample and the nucleic acid - protein complexes formed during the purification process. There is a certain degree of stickiness between nucleic acids and proteins in biological samples.

The traditional nucleic acid removal methods have the following problems:

BioSMNE is a kind of endonuclease derived from Serratia marcescens and is a genetically engineered and site-directed mutagenized highly specific endonuclease, also known as "omnipotent nuclease". It can efficiently degrade all forms of nucleic acids, including single-stranded, double-stranded, circular, and linear DNA/RNA. It can specifically degrade them into 3-7 nucleotide oligonucleotides. BioSMNE has a high affinity for protein products, ensuring the integrity of proteins during the purification process. While BioSMNE efficiently degrades nucleic acids, it also has other advantages, such as reducing sample viscosity, ensuring protein activity, and having high stability in a wide range of pH and temperature conditions, making it more suitable for various protein purification processes.

We provide high-purity and high-activity BioSMNE to help customers solve the impact of nucleic acid impurities on protein purification, including:

• 1            

  Completely remove nucleic acid impurities

• 2            

  Reduce sample viscosity, improve protein yield

• 3            

  Minimize the impact of exogenous nucleases on the activity and integrity of target proteins

• 4            

  Improve the separation efficiency of proteins and increase the resolution of protein separation