Workflow and Q&A
One-stop Service (From Gene to Structure)
Structural biology plays a crucial role in various stages of new drug development. Biortus has successfully established a one-stop CRO service for structural biology, covering the entire workflow from gene synthesis, protein expression, and protein preparation to bioanalysis, X-ray crystallography, and cryo-electron microscopy (cryo-EM) structure determination. As innovative drug development continues to advance and accelerate, structural biology will play an increasingly important role. Biortus will continue to deepen its expertise in structural biology services, providing high-quality and efficient services to customers both domestically and internationally. Additionally, with the goal of delivering "source innovation," the company is committed to building the "Thousand Targets, Ten Thousand Leads" platform, which is expected to provide a continuous source of innovative momentum for customers worldwide.
Q&A
Is there a molecular weight limit for cryo-EM single particle analysis?
Generally, the molecular weight of the target should be greater than 70 kDa. For proteins with a molecular weight less than 70 kDa, further evaluation is required to determine their suitability for SPA.
What is the turnaround time for cryo-EM single particle analysis?
Under the condition of stable protein samples, it typically takes 2 to 4 weeks of turnaround time to optimize cryo-EM sample preparation conditions and resolve the structure. If the sample does not require optimization, results can be delivered in as little as 1 to 2 weeks.
What resolution can be achieved with cryo-EM single particle analysis?
The final resolution depends on the structural characteristics of the protein itself. The higher the structural rigidity and homogeneity, the better the quality of the structure resolution. For example, the standard sample apo-ferritin can be resolved to 1.5 angstrom, while typical pharmaceutical target proteins are generally resolved to around 3 angstrom.
Cryo-EM Structure Gallery
