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InAS2/ImS2 Systems

Product Introduction

ImS2TM Bioarose and ImS2TMcolumn can quickly separate and obtain InAS2 tag recombinant proteins

ImS2 Bioarose is an affinity chromatography filler used to purify recombinant proteins with InAS2 tags. These recombinant proteins can specifically bind to the filler with ImS2 ligands, achieving high-purity protein affinity purification. Compared with traditional affinity fillers, it has higher affinity and specificity, can tolerate a wider range of purification conditions, and can obtain high-purity proteins through one-step purification.

Advantages of ImS2 Bioarose

● One-step purification of high-purity InAS2 tag recombinant proteins.

● Mild elution conditions to ensure protein activity.

● Simple regeneration method.

● Compatible with various additives such as detergents, reductants, and metal chelators.

● Available in 5mL, 10mL, and 50mL filler packages, as well as 1mL and 5mL ImS2 pre-packed columns.

ImS2 Introduction

The ligand on the ImS2 filler is modified from the ImS2 protein of Pseudomonas aeruginosa and has a picomolar (pM) level of high affinity with the InAS2 tag.

The InAS2 tag originates from a small protein, pyoS2, that binds to ImS2 and has DNase activity. Biortus modified it to lose its DNase activity without changing its affinity for ImS2.

ImS2 column characteristics

ImS2 column is a column filled with ImS2 Bioarose. The pre-packed column shell is made of polypropylene material that does not interact with biological molecules, and provides two specifications of 1ml and 5ml pre-packed columns for the convenience of laboratory personnel for purification.

ImS2 characteristics

  • Matrix

    4% cross-linked agarose

  • Particle size

    45-165 μm

  • Ligand

    ImS2

  • Ligand conc.

    ~15 mg/mL resin

  • Binding capacity

    ~35 mg of 50 KD target protein/mL resin

  • Flow rate

    ≤150 cm/h

  • Max pressure

    0.3 MPa (3 bar, 45 psi)

  • pH

    pH 5-10

  • Storage

    2-8 ºC, 20% ethanol

Compatibility of ImS2 Bioarose with different additives

  • NaCl

    2M

  • EDTA

    10 mM

  • TritonX-100

    2%

  • NP-40

    2%

  • β-Mercaptoethanol

    45 mM

  • DTT

    5 mM

  • Imidazole

    300 mM

  • Ethanol

    20%

Ordering Information

Catalog No. Products Specification
MS39140-01R ImS2 Bioarose 1 mL
MS39140-05R ImS2 Bioarose 5 ml
MS39140-10R ImS2 Bioarose 10 mL
MS39140-50R ImS2 Bioarose 50 mL
MS39140-11C ImS2 column 1 x 1 mL
MS39140-51C ImS2 column 5 x 1 mL
MS39140-15C ImS2 column 1 x 5 mL
MS39140-55C ImS2 column 5 x 5 mL

Experimental Tools

Experimental Tools