BP08513-01A-Biortus Biosciences Co. Ltd.

BP08513-01A

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Family ：

E3 ubiquitin-protein ligase

Catalog No. ：

BP08513-01A

Protein ：

SMURF2

UniProt ：

Q9HAU4

Organism ：

Homo sapiens

Expression system ：

E. coli

Tag ：

His

Sequence ：

S2-E748

Inquiry ：

是

Symbol/Gene Name: SMURF2

Alias: E3 ubiquitin-protein ligase SMURF2,hSMURF2,HECT-type E3 ubiquitin transferase SMURF2,SMAD ubiquitination regulatory factor 2,SMAD-specific E3 ubiquitin-protein ligase 2

Uniprot: Q9HAU4

Background: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:11016919). Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling (PubMed:11163210, PubMed:12717440). In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1 (PubMed:18448069). Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation (PubMed:11016919, PubMed:11158580, PubMed:11389444). SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation (PubMed:11389444). Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation (PubMed:30696809).

Construct: 6His-TEV-(GG)-SMURF2(S2-E748 end)

Expression Host: E. coli

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Recombinant protein

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