BP12397-02A-Biortus Biosciences Co. Ltd.

BP12397-02A

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Family ：

E3 ubiquitin-protein ligase

Catalog No. ：

BP12397-02A

Protein ：

RNF114

UniProt ：

Q9Y508

Organism ：

Homo sapiens

Expression system ：

E. coli

Tag ：

GST

Sequence ：

A2-Q228

Inquiry ：

是

Symbol/Gene Name: RNF114

Alias: E3 ubiquitin-protein ligase RNF114,RING finger protein 114,RING-type E3 ubiquitin transferase RNF114,Zinc finger protein 228,Zinc finger protein 313

Uniprot: Q9Y508

Background: E3 ubiquitin-protein ligase that promotes the ubiquitination of various substrates (PubMed:23645206, PubMed:25165885). In turn, participates in the regulation of many biological processes including cell cycle, apoptosis, osteoclastogenesis as well as innate or adaptive immunity (PubMed:25165885, PubMed:28708287). Acts as negative regulator of NF-kappa-B-dependent transcription by promoting the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3 (PubMed:25165885). May promote the ubiquitination of TRAF6 as well (PubMed:28708287). Acts also as a negative regulator of T-cell activation (PubMed:25165885). Inhibits cellular dsRNA responses and interferon production by targeting MAVS component for proteasomal degradation (PubMed:25165885). Ubiquitinates the CDK inhibitor CDKN1A leading to its degradationand probably also CDKN1B and CDKN1C (PubMed:23645206). This activity stimulates cell cycle G1-to-S phase transition and suppresses cellular senescence. May play a role in spermatogenesis.

Construct: GST-TEV-GG-RNF114(A2-Q228 end)

Expression Host: E. coli

Purity: 90%

Formulation: 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Recombinant protein

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