BP471A2-24A-Biortus Biosciences Co. Ltd.

BP471A2-24A

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Family ：

CDK

Catalog No. ：

BP471A2-24A

Protein ：

CDK4/Cyclin D2

UniProt ：

P11802/P30279

Organism ：

Homo sapiens

Expression system ：

Insect cell

Tag ：

GST/Flag/His

Sequence ：

M1-E303/E2-L289

Inquiry ：

是

Symbol/Gene Name: CDK4;CCND2

Alias: Cyclin-dependent kinase 4,Cell division protein kinase 4,PSK-J3;G1/S-specific cyclin-D2

Uniprot: P11802/P30279

Background: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed:8114739, PubMed:18827403). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed:8114739, PubMed:18827403). Hypophosphorylates RB1 in early G(1) phase (PubMed:8114739, PubMed:18827403). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed:8114739, PubMed:18827403).

Construct: N-GST-3C-CDK4(1-303end)/pFastBac1-Flag-8His-TEV-Cyclin D2(E2-L289 end)

Expression Host: Insect cell

Purity: 90%

Formulation:

Supplied as sterile 50 mM HEPES (pH 7.5), 150 mM NaCl, 5% glycerol.

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Recombinant protein

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