Kinase Assays
Capillary electrophoretic separation prior to fluorescence detection in a Caliper LabChip mobility shift assay offers improved assay reliability and extends assay applicability to mixed samples. The direct, quantifiable and real time monitoring of substrate and product level makes it particularly useful for enzyme assays. For example, the activity of a kinase could be measured by monitoring the formation of a phosphorylated fluorescently labelled peptide.