BP02577-12A

Family :

Protease and Enzyme

Catalog No. :

BP02577-12A

Protein :

TEV Protease

UniProt :

P04517

Organism :

Tobacco etch virus

Expression system :

E. coli

Tag :

His/GST

Sequence :

G2038-N2273

Inquiry :

Symbol/Gene Name:  Tobacco etch virus (TEV)

Alias:  Genome polyprotein

Uniprot: P04517

Background: Required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus (PubMed:2656254). Interacts with virions and aphid stylets (PubMed:9880030). Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs (PubMed:11414807). May have RNA-binding activity.

Construct: 6His-GST-Thrombin-TEV Protease(2038-2273)(S2256V)-5R-GGSC

Expression Host: E. coli

Purity: 80%

Formulation: 50mM Tris7.5, 200mM NaCl, 20%glycerol, 1mM DTT.

Supplied as sterile

Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hard copy of CoA.

Endotoxin:

< 0.01 EU per μg of the protein

Shipping:

Liquid. It is shipped out with blue ice.

Stability & Storage:

Samples are stable for up to twelve months from date of receipt at -20 to -80

Store it under sterile conditions at -20 to -80. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

 

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